Phylogenetic status and phosphate solubilizing ability of Circinella muscae (Mucorales, Mucoromycota), isolated from soil, in India

Kajol Yadav, Jitendra R. Patil, Thalline Rafhaella Leite Cordeiro, Andre L.C.M. de A. Santiago & Lal Sahab Yadav

Sydowia 78: 65-74

Published online on May 20th, 2025

During an exploration of fungi from soil in Maharashtra, India, a Mucorales strain CHMC-02 was isolated from the botanical garden soil of CHM College, Ulhasnagar, Maharashtra, India. Based on morphological characteristics and sequence analysis of two genes, ITS1-5.8S-ITS4 and 28SrDNA (LSU) the isolate was confirmed to be Circinella muscae in the family Circinellaceae. The strain CHMC-02 demonstrated optimum growth at 28°C, at pH between 5 and 6 on PDA medium. Additionally, a study of its phosphate solubilizing ability showed excellent phosphate solubilization potential with solubilizing index (2.25) on Pikovskaya’s agar plate and also very high phosphate solubilizing ability (342 µg ml–1) in Pikovskaya’s broth after seven days of incubation. The screening of acid resistance study showed that the strain CHMC-02 can grow well at pH ranging from 2.5 to 7.5. To the best of our knowledge, this is the first report of phosphate solubilization by the C. muscae.

Keywords: Circinellaceae, Mucorales, multigenes, phosphate solubilizing index

eBook at Verlag Berger